@Article{	  Donesz-Sikorska2014,
  author	= {Donesz-Sikorska, Anna and Grzesiak, Jakub and Śmieszek, Agnieszka and Krzak, Justyna and Marycz, Krzysztof},
  title		= {The influence of sol-gel-derived silica coatings functionalized with betamethasone on adipose-derived stem cells (ASCs)},
  journal	= {Journal of Biomaterials Applications},
  year		= {2014},
  pages		= {1-12},
  abstract	= {Silica-based sol–gel coatings have gained attention in bone therapies and orthopedic applications, due to the biocompatibility and bioactivity, including a
		  high potential for the controlled release both in vitro and in vivo. Bioactive materials are created to facilitate the biocompatibility of orthopedic implants.
		  One of the promising alternatives is biomaterials with immobilized drugs. In this study we demonstrated for the first time novel sol–gel-derived silica coatings
		  with active amino groups (SiO2(NH2)) functionalized with a steroid drug—betamethasone, applied to a substrate 316 L using dip coating technique. The presence
		  of betamethasone in functionalized coatings was directly confirmed by Raman spectroscopy and energy-dispersive X-ray spectroscopic analysis. The wettability was
		  evaluated by the sessile drop method, while the surface free energy was estimated based on the contact angles measured. Our results showed a shift in surface
		  properties from hydrophobic to hydrophilic after application of the coatings. We have investigated the morphology, proliferation factor, and the population
		  doubling time of adipose-derived stem cells for biological purposes. Moreover, the analysis of the distribution and localization of cellular microvesicles was
		  performed to evaluate the influence of functionalized surfaces on cellular cytophysiological activity. Increased proliferation and activation of cells, determined
		  by the observations of microvesicles shedding processes, provided evidence of the availability of the drug. Therefore, we conclude that the sol–gel synthesis
		  proposed here allows to improve the metal substrates and can be successfully used for immobilization of betamethasone. This in turn enables the direct delivery of
		  the drug with implanted material into the wound site, and to stimulate the activity of cells to enhance tissue regeneration.},
  doi		= {10.1177/0885328214534003},
  keywords	= {powłoki zol-żelowe, tłuszczowe komórki macierzyste, betametazon, implanty metaliczne, medycyna regeneracyjna},
  url		= {http://jba.sagepub.com/content/early/2014/05/15/0885328214534003.full.pdf+html}
}